Jianghong Rao Lab In the Departments of Radiology and Chemistry

Kanyi Pu, Ph.D.

Kanyi Pu, Ph.D.

Kanyi’s research in the Rao Lab mainly focuses on the development of biocompatible polymer nanoparticles for molecular imaging of disease-related microenvironment.

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08/2007 – 05/2011
Ph. D., Department of Chemical and Biomolecular Engineering
Faculty of Engineering
National University of Singapore (NUS), Singapore

09/2004 – 06/2007
M. S., Polymer Chemistry and Physics
Department of Macromolecular Science
Institute of Advanced Materials
Fudan University (FDU), China

09/2000 – 06/2004
B. E., Materials Physics
Department of Polymer Science
School of Materials Science and Engineering
East China University of Science and Technology (ECUST), China


    1. Pu K,* Shuhendler AJ,* Valta MP,*, Cui L, Saar M, Peehl DM, Rao J. Phosphorylcholine-Coated Semiconducting Polymer Nanoparticles as Rapid and Efficient Labeling Agents for In Vivo Cell Tracking. Adv Healthc Mater. 2014 Mar 25. doi: 10.1002/adhm.201300534. *Co-first author
    2. Shuhendler AJ,* Pu K,*, Cui L, Uetrecht JP, Rao J. Real-time imaging of oxidative and nitrosative stress in the liver of live animals for drug-toxicity testing. Nat Biotechnol. 2014 Mar 23. doi: 10.1038/nbt.2838. *Co-first author
    3. Pu K,* Shuhendler AJ,*, Jokerst JV, Mei J, Gambhir SS, Bao Z, Rao J. Semiconducting polymer nanoparticles as photoacoustic molecular imaging probes in living mice. Nat Nanotechnol. 2014,9, 233-239. *Co-first author
    4. Pu K, Shuhendler AJ, and Rao J. Semiconducting Polymer Nanoprobe for In Vivo Imaging of Reactive Oxygen and Nitrogen Species . Angew. Chem. Int. Ed. 2013. doi: 10.1002/anie.201303420
    5. Wang LH*, Pu KY*, Jing L, Qi XY, Li H, Zhang H, Fan CH, and B. Liu. A graphene/conjugated oligomer hybrid sensor for light-up sensing of lectins and bacteria. Adv. Mater. 2011, 23, 4386–4391. *Co-first author
    6. Pu KY and Liu B. Florescent Conjugated Polyelectrolytes for Bioimaging. Adv. Funct. Mater. 2011, 21, 3408–3423.
    7. Pu KY, Shi, JB, Cai LP, Li K, and Liu B. Affibody-attached hyperbranched conjugated polyelectrolyte for targeted fluorescence imaging of HER2-positive cancer cell. Biomacromolecules 2011, 12, 2966–2974.
    8. Pu KY, Luo ZT, Li K, Xie JP, and Liu B. Energy transfer between gold nanocluster and conjugated oligoelectrolyte substituted POSS for multicolor intracellular detection of mercury ion. J. Phys. Chem. C 2011, 115, 13069–13075.
    9. Pu KY, Zhan RY, Liang J, and Liu B. Conjugated polyelectrolyte for label-free visual detection of heparin. Sci. China Ser. B–Chem. 2011, 54, 567–574.
    10. Pu KY, Li K, Zhang XH, and Liu B. Conjugated oligoelectrolyte harnessed POSS as light-up hybrid unimolecular nanodots for two-photon fluorescence imaging of cellular nucleus. Adv. Mater. 2010, 22, 4186–4189.
    11. Pu KY, Li K, and Liu B. Cationic oligofluorene substituted POSS as light-harvesting unimolecular nanoparticle for fluorescence amplification in cellular imaging. Adv. Mater. 2010, 22, 643–646.
    12. Pu KY, Li K, and Liu B. A molecular brush approach to enhance quantum yield and suppress nonspecific interactions of conjugated polyelectrolyte for far-red/near- infrared fluorescence targeted cell imaging. Adv. Funct. Mater. 2010, 20, 2770–2777.
    13. Pu KY, Li K, and Liu B. Multicolor conjugate polyelectrolyte/peptide complexes as self- assembled nanoparticles for receptor-targeted cellular imaging. Chem. Mater. 2010, 22, 6736–6741.
    14. Pu KY, Zhan RY, and Liu B. Conjugated polyelectrolyte blend as perturbable energy donor- acceptor assembly for multicolor fluorescence responses to proteins. Chem. Commun. 2010, 46, 1470–1472.
    15. Pu KY, Shi JB, Wang LH, Cai LP, Wang G, and Liu B. Mannose-substituted conjugated polyelectrolyte and oligomer as an intelligent energy transfer pair for visual detection of concanavalin A. Macromolecules 2010, 43, 9690–9697.
    16. Pu KY and Liu B. Fluorescence turn-on responses of anionic and cationic conjugated polymers toward proteins: effect of electrostatic and hydrophobic interactions. J. Phys. Chem. B 2010, 114, 3077–3084.
    17. Pu KY and Liu B. Intercalating dye harnessed cationic conjugated polymer for real-time naked-eye recognition of double-stranded DNA in serum. Adv. Funct. Mater. 2009, 19, 1371–1378.
    18. Pu KY and Liu B. Conjugated polyelectrolytes as light-up macromolecular probes for heparin sensing. Adv. Funct. Mater. 2009, 19, 277–284.
    19. Pu KY and Liu B. Optimizing the cationic conjugated polymer-sensitized fluorescent signal of dye labeled oligonucleotide for biosensor applications. Biosens. Bioelectron. 2009, 24, 1067–1073.
    20. Pu KY, Li K, Shi, JB, and Liu B. Fluorescent single-molecular core-shell nanospheres of hyperbranched conjugated polyelectrolyte for live-cell imaging. Chem. Mater. 2009, 21, 3816–3822.
    21. Pu KY, Cai LP, and Liu B. Design and synthesis of charge-transfer-based conjugated polyelectrolytes as multicolor light-up probes. Macromolecules 2009, 42, 5933–5940.
    22. Pu KY, Fang, Z, and Liu B. Effect of charge density on energy-transfer properties of cationic conjugated polymers. Adv. Funct. Mater. 2008, 18, 1321–1328.
    23. Pu KY and Liu B. A multicolor cationic conjugated polymer for naked-eye detection and quantification of heparin. Macromolecules 2008, 41, 6636–6640.
    24. Pu KY, Pan SYH, and Liu B. Optimization of interactions between a cationic conjugated polymer and chromophore-labeled DNA for optical amplification of fluorescent sensors. J. Phys. Chem. B 2008, 112, 9295–9300.
    25. Pu KY, Qi XY, Lu XM, Yang LL, Li TC, Fan QL, Liu B, Chan HSO, Wang C, and Huang W. Supramolecular regulated photophysical properties of oligo(p-phenyleneethynylene)- base rod-coil block copolymers: effect of molecular architecture. Chem. Eur. J. 2008, 14, 1205–1215.

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